PHYTOCHEMICAL ANALYSIS AND CHARACTERIZATION OF COMPOUNDS RESPONSIBLE FOR IN-VITRO CYTOTOXIC ACTIVITY OF EUPATORIUM ODORATUM LEAF (LIN)

OKONKWO, SYLVIA IFEYINWA PhD

FEDERAL UNIVERSITY OF TECHNOLOGY OWERRI

 

 

The medicinal plant Eupatorium odoratum leaves of the family asteracae, which was claimed to have medicinal values was analysed phytochemically and characterization of the compounds responsible for in-vitro cytotoxic activities was also carried out. The phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, terpenoids. The crude and pure extracts from the leaves were purified, identified and characterized by using thin layer chromatography, column chromatography, elemental analyzer, mass spectrometry, nuclear magnetic resonance spectra and infrared spectra. Ten pure substances were isolated and characterized using IR, NMR, elemental, molecular ion spectroscopy were established. These are: 5,7-dihydroxy-4H-chromen-4-one, 3,5,7-trihydroxy – 4H – chromen -4-one, 5,7-dihydroxy-2-methoxy-4H-chromen-4-one, 2-(3,4-dihydroxyphenyl)-5-hydroxy-7-(3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yloxy)-4a,8a-dihydro-2H-chrome-4(3H)-one, 2-isopropyl-5-methyl cyclohexanone, 2s-methyl-5-(4-methylpentyl)-3,4,4a,5,6,7,8,8a-octahydronaphthalen-1-yl)methanol, 1-isopropyl-4,7-dimethyl-1,5,4a,5,8,8a-hexahydronaphthalene, 3,7,11,15-tetramethylhexadecan-1-ol, (1S)-7-acetoxy-2,3,5,7a-tetrahydro-1H-pyrrolizin-1-y1 methacrylate and (6R, 14aS,Z)-3-ethylidene-6-hydroxy-5,6-dimethyl-3,4,5,6,11,13,14,14a-octahydro [1,6] dioxacyclododeca [2,3,4-gh] pyrrolizine-2,7 (9H, 9a1H) – dione.

Crude extracts from the leaves exhibited 60% inhibitory effects on four types of bacteria cultures while the isolated compounds showed 10% on a replicate bacteria cultures.

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